Functional analysis of a pathogenesis-related thaumatin-like protein gene TaLr35PR5 from wheat induced by leaf rust fungus

Functional analysis of a pathogenesis-related thaumatin-like protein gene TaLr35PR5 from wheat induced by leaf rust fungus

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Abstract Background Plants have evolved multifaceted defence mechanisms to resist pathogen infection.Production of the pathogenesis-related (PR) proteins in response to pathogen attack has been implicated in plant disease resistance specialized in systemic-acquired resistance (SAR).Our earlier studies have reported that a full length TaLr35PR5 gene, encoding a protein exhibiting amino acid and structural similarity to a sweet maidenform self expressions strapless bra protein thaumatin, was isolated from wheat near-isogenic line TcLr35.

The present study aims to understand the function of TaLr35PR5 gene in Lr35-mediated adult resistance to Puccinia triticina.Results We determined that the TaLr35PR5 protein contained a functional secretion peptide by utilizing the yeast signal sequence trap system.Using a heterologous expression assay on onion two tone woven basket epidermal cells we found that TaLr35PR5 protein was secreted into the apoplast of onion cell.

Expression of TaLr35PR5 was significantly reduced in BSMV-induced gene silenced wheat plants, and pathology test on these silenced plants revealed that Lr35-mediated resistance phenotype was obviously altered, indicating that Lr35-mediated resistance was compromised.Conclusions All these findings strongly suggest that TaLr35PR5 is involved in Lr35-mediated adult wheat defense in response to leaf rust attack.